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Dynamic changes in glycosylation and glycan composition of serum FSH and LH during natural ovarian stimulation
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Biochemial structure and function.
2013 (English)In: Upsala Journal of Medical Sciences, ISSN 0300-9734, E-ISSN 2000-1967, Vol. 118, no 3, 153-164 p.Article in journal (Refereed) Published
Abstract [en]

Background. Glycosylation and glycan composition are of fundamental importance for the biological properties of FSH and LH. The aim of this study was to determine the glycosylation, sialylation, and sulfonation of serum FSH and LH throughout the normal menstrual cycle. Methods. Serum samples were collected from 79 healthy women with regular menstrual cycles. The mean numbers of anionic monosaccharide (AMS), sialic acid (SA), and sulfonated N-acetylgalactosamine (SU) residues per FSH and LH molecule were estimated for all sera with methods based on electrophoreses, neuraminidase treatments, and fluoroimmunoassays of the gonadotrophins. Results. Di-glycosylated glycoforms (FSHdi, LHdi) were detected in serum in addition to tetra-glycosylated FSH (FSHtetra) and tri-glycosylated LH (LHtri). FSHdi exhibited two peaks: one on day 5 to 7 and one, more pronounced, at midcycle. FSHtetra plateaued at a high concentration from day 5 to 15, without a midcycle peak. There were lower concentrations of LHdi than LHtri, except at midcycle when the opposite occurred. The mean numbers of SA and SU residues per molecule of FSH and LH in serum showed four different patterns during the cycle, all with highly significant (P < 0.0001) differences between levels at different phases of the cycle. The pattern of SA residues on FSH was 'M'-shaped, and that of SU on LH 'V'-shaped. Conclusion. Serum FSH and LH governing the natural ovarian stimulation process exhibited dynamic changes of glycosylation and glycan composition. This new information on the FSH and LH molecular structures may lead to more successful mono-ovulatory treatment regimens for ovulation induction in anovulatory women.

Place, publisher, year, edition, pages
2013. Vol. 118, no 3, 153-164 p.
Keyword [en]
di-glycosylated FSH, di-glycosylated LH, glycoforms, isoforms, ovulation induction, sialic acid, sulfonated N-acetylgalactosamine
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-204851DOI: 10.3109/03009734.2013.782081ISI: 000321587100002OAI: oai:DiVA.org:uu-204851DiVA: diva2:640137
Available from: 2013-08-12 Created: 2013-08-12 Last updated: 2017-12-06Bibliographically approved

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Wide, LeifEriksson, Karin

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