Expression of chondroitin/dermatan sulfate glycosyltransferases during early zebrafish development
2013 (English)In: Developmental Dynamics, ISSN 1058-8388, E-ISSN 1097-0177, Vol. 242, no 8, 964-975 p.Article in journal (Refereed) Published
Background: Chondroitin/dermatan sulfate (CS/DS) proteoglycans present in the extracellular matrix have important structural and regulatory functions. Results: Six human genes have previously been shown to catalyze CS/DS polymerization. Here we show that one of these genes, chpf, is represented by two copies in the zebrafish genome, chpfa and chpfb, while the other five human CS/DS glycosyltransferases csgalnact1, csgalnact2, chpf2, chsy1, and chsy3 all have single zebrafish orthologues. The putative zebrafish CS/DS glycosyltransferases are spatially and temporally expressed. Interestingly, overlapping expression of multiple glycosyltransferases coincides with high CS/DS deposition. Finally, whereas the relative levels of the related polysaccharide HS reach steady-state at around 2 days post fertilization, there is a continued relative increase of the CS amounts per larvae during the first 6 days of development, matching the increased cartilage formation. Conclusions: There are 7 CS/DS glycosyltransferases in zebrafish, which, based on homology, can be divided into the CSGALNACT, CHSY, and CHPF families. The overlap between intense CS/DS production and the expression of multiple CS/DS glycosyltransferases suggests that efficient CS/DS biosynthesis requires a combination of several glycosyltransferases.
Place, publisher, year, edition, pages
2013. Vol. 242, no 8, 964-975 p.
chondroitin sulfate, polymerase, CSGALNACT, CHSY, CHPF, zebrafish
IdentifiersURN: urn:nbn:se:uu:diva-204834DOI: 10.1002/dvdy.23981ISI: 000321843400008OAI: oai:DiVA.org:uu-204834DiVA: diva2:640333
De två (2) första författarna delar förstaförfattarskapet.2013-08-132013-08-122015-11-10Bibliographically approved