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A point mutation downstream of the -10 promoter element does not exhibit long-range effect on TetR binding
(English)Manuscript (preprint) (Other academic)
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-205306OAI: oai:DiVA.org:uu-205306DiVA: diva2:641190
Available from: 2013-08-15 Created: 2013-08-15 Last updated: 2015-06-22
In thesis
1. Promoter Engineering for Cyanobacteria: An Essential Step
Open this publication in new window or tab >>Promoter Engineering for Cyanobacteria: An Essential Step
2013 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Synthetic biology views a complex biological system as an ensemble in the hierarchy of parts, devices, systems, and networks. The practice of using engineering rules such as decoupling and standardization to understand, predict, and re-build novel biological functions from model-driven designed genetic circuits is emphasized. It is one of the top ten technologies that could help solving the current and potential risks in human society. Cyanobacteria have been considered as a promising biological system in conducting oxygenic photosynthesis to convert solar energy into reducing power, which drives biochemical reactions to assimilate and generate chemicals for a specific purpose such as CO2 fixation, N2 fixation, bioremediation, or fuels production. The promoter is a key biological part to construct feedback loops in genetic circuits for a desired biological function. In this thesis, promoters that don't work in the cyanobacterium Synechocystis PCC 6803 in terms of promoter strength, and dynamic range of gene regulation are identified. Biological parts, such as ribosome binding sites, and reporter genes with and without protease tags were also characterized with the home-built broad-host-range BioBrick shuttle vector pPMQAK1. The strong L03 promoter, which can be tightly regulated in a wide dynamic range by the foreign Tet repressor, was created through an iterative promoter engineering cycle. The iteration cycle of DNA breathing dynamic simulations and quantification of a reporting signal at a single-cell level should guide through the engineering process of making promoters with intended regulatory properties. This thesis is an essential step in creating functional promoters and it could be applied to create more diverse promoters to realize the emphasized practices of synthetic biology to build synthetic cyanobacteria for direct fuel production and CO2 assimilation.

Place, publisher, year, edition, pages
Acta Universitatis Upsaliensis, 2013. 58 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1063
Keyword
synthetic biology, cyanobacteria, promoter, engineering, TetR, DNA breathing dynamics, transcription, regulation
National Category
Biochemistry and Molecular Biology
Identifiers
urn:nbn:se:uu:diva-206901 (URN)978-91-554-8724-9 (ISBN)
Public defence
2013-09-27, Polacksbacken, Pol_2146, Lägerhyddsvägen 1, Uppsala, 13:15 (English)
Opponent
Supervisors
Available from: 2013-09-06 Created: 2013-09-06 Last updated: 2015-07-29Bibliographically approved

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Seeger, Christian

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