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Enhanced interpretation of adsorption data generated by liquid chromatography and by modern biosensors
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry.
Department of Engineering and Chemical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden.
Department of Engineering and Chemical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Chemistry, Department of Chemistry - BMC, Analytical Chemistry. Department of Engineering and Chemical Sciences, Karlstad University, SE-651 88 Karlstad, Sweden.
2013 (English)In: Journal of Chromatography A, ISSN 0021-9673, E-ISSN 1873-3778, Vol. 1317, no SI, 22-31 p.Article in journal (Refereed) Published
Abstract [en]

In this study we demonstrate the importance of proper data processing in adsorption isotherm estimations. This was done by investigating and reprocessing data from five cases on two closely related platforms: liquid chromatography (LC) and biosensors. The previously acquired adsorption data were reevaluated and reprocessed using a three-step numerical procedure: (i) preprocessing of adsorption data, (ii) adsorption data analysis and (iii) final rival model fit. For each case, we will discuss what we really measure and what additional information can be obtained by numerical processing of the data. These cases clearly demonstrate that numerical processing of LC and biosensor data can be used to gain deeper understanding of molecular interactions with adsorption media. This is important because adsorption data, especially from biosensors, is often processed using old and simplified methods.

Place, publisher, year, edition, pages
2013. Vol. 1317, no SI, 22-31 p.
Keyword [en]
Surface Plasmon Resonance, Quartz Crystal Microbalance, Data processing, Fluorescence, Adsorption isotherm, Adsorption energy distribution
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:uu:diva-205628DOI: 10.1016/j.chroma.2013.07.077ISI: 000327229600004OAI: oai:DiVA.org:uu-205628DiVA: diva2:642254
Available from: 2013-08-21 Created: 2013-08-21 Last updated: 2017-12-06Bibliographically approved

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Publisher's full texthttp://www.sciencedirect.com/science/article/pii/S0021967313011564

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Agmo Hernández, VíctorFornstedt, Torgny

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