Effects of combined use of nicotine and ethanol on human neuroblastoma cells
Independent thesis Advanced level (degree of Master (Two Years)), 20 credits / 30 HE creditsStudent thesis
According to the Swedish Public Health Institute, around 2 million swedes use some kind of tobacco product regularly. 90 % of the Swedish people also report that they drink alcohol. That nicotine and ethanol are often used together is a well-known fact and it seems that these drugs tend to increase the use of each other. Both nicotine and ethanol has shown both neuroprotective and neurotoxic properties in previous studies, depending on the doses used. Combination of low-dose ethanol and nicotine has, in contrast to some hypotheses, pointed towards a synergistic effect and an enhanced toxicity. Hence, the aim of this study was to investigate how a combined use of these substances could affect human neuroblastoma cells (SH-SY5Y). The cells were seeded onto 96 well plates and treated with different concentrations of ethanol, staurosporine or hydrogen peroxide. Staurosporine was used as a neurotoxic reference. A pre-treatment approach was also studied, where nicotine was added to the cells 24 hours prior to neurotoxic agent. The cell viability was measured using an MTT assay 48 hours later. The results showed that nicotine was unable to protect the cells against staurosporine toxicity, but a tendency of decreased toxicity was observed in cells treated with hydrogen peroxide. Concomitant administration of nicotine and ethanol showed a decreased viability compared to nicotine and ethanol alone, which was in line with previous studies. Pre-treatment with nicotine reduced the toxic effects, but the cell viability was still lowered compared to control. Since this study is based on a small number of repeats of each experiment, further research is needed to be able to draw conclusions about both the results and the mechanisms behind them. However, signs indicate that the concomitant use of ethanol and nicotine may induce a stronger neurotoxicity than if they are used separately.
Place, publisher, year, edition, pages
Pharmacology and Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-205887OAI: oai:DiVA.org:uu-205887DiVA: diva2:642958
Subject / course
Master of Science Programme in Pharmacy