Theoretical study of the accuracy of the elution by characteristic points method for bi-Langmuir isotherms
2001 (English)In: Journal of Chromatography A, ISSN 0021-9673, Vol. 908, no 1-2, 111-130 p.Article in journal (Refereed) Published
The bi-Langmuir equation has recently been proven essential to describe chiral chromatographic surfaces and we therefore investigated the accuracy of the elution by characteristic points method (ECP) for estimation of bi-Langmuir isotherm parameters. The ECP calculations was done on elution profiles generated by the equilibrium-dispersive model of chromatography for five different sets of bi-Langmuir parameters. The ECP method generates two different errors; (i) the error of the ECP calculated isotherm and (ii) the model error of the fitting to the ECP isotherm. Both errors decreased with increasing column efficiency. Moreover, the model error was strongly affected by the weight of the bi-Langmuir function fitted. For some bi-Langmuir compositions the error of the ECP calculated isotherm is too large even at high column efficiencies. Guidelines will be given on surface types to be avoided and on column efficiencies and loading factors required for adequate parameter estimations with ECP.
Place, publisher, year, edition, pages
2001. Vol. 908, no 1-2, 111-130 p.
adsorption isotherms; elution by characteristic point method; PREPARATIVE LIQUID-CHROMATOGRAPHY; PROPRANOLOL ENANTIOMERS; BAND; SEPARATIONS; PROPAGATION; SIMULATION; CELLULASE
IdentifiersURN: urn:nbn:se:uu:diva-36545DOI: 10.1016/S0021-9673(00)00965-1PubMedID: 11218114OAI: oai:DiVA.org:uu-36545DiVA: diva2:64444