The Suspended Formalin Test:: A Method Designed for Studying Formalin-Induced Behaviour in the Speke's Hingeback Tortoise (Kinixys spekii)
2012 (English)In: Scandinavian Journal of Laboratory Animal Science, ISSN 0901-3393, Vol. 39, no 1, 11-15 p.Article in journal (Refereed) Published
The present study aimed to develop a method for testing pain-related behaviour induced by formalin in theSpeke’s hingeback tortoise (Kinixys spekii). These animals retract their head and limbs into their shell whenapproached, making behavioural testing almost impossible. It was found that suspending the animals in theair, facing away from the observer, made the animals keep their limbs out of the shell. Subcutaneous injectionof formalin induced easily identifiable and quantifiable behaviours that lasted for 20 minutes. Contraryto the biphasic effect of formalin observed in rats and mice, the response in tortoises was monophasic. Thesuspended formalin test may be useful for studying nociceptive mechanisms in tortoises, which in turn willbe important for a further understanding of the nociceptive system in reptiles as well as in mammals.
Place, publisher, year, edition, pages
2012. Vol. 39, no 1, 11-15 p.
IdentifiersURN: urn:nbn:se:uu:diva-206526OAI: oai:DiVA.org:uu-206526DiVA: diva2:644621