uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Effects of di- and polysaccharide formulations and storage conditions on survival of freeze-dried Sphingobium sp.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmacy.
Show others and affiliations
2013 (English)In: World Journal of Microbiology & Biotechnology, ISSN 0959-3993, E-ISSN 1573-0972, Vol. 29, no 8, 1399-1408 p.Article in journal (Refereed) Published
Abstract [en]

In this study we have compared the ability of the organic polymers Ficoll and hydroxyethylcellulose (HEC) and the disaccharides sucrose and trehalose to support cell survival during freeze-drying and subsequent storage of a gram-negative Sphingobium sp. In addition to determination of viability rates, cell integrity was evaluated using lipid peroxidation and RNA quality assays for the different storage conditions and formulation compositions. All formulations resulted in high initial cell survival rates after freeze-drying. However, the disaccharide formulations were superior to the polymer-based formulations in supporting cell survival during storage with the exception of Ficoll that upon storage under vacuum yielded bacterial survival rates equal to that of sucrose. Storage in the presence of both oxygen and moisture was detrimental for bacterial survival in all formulations tested, however, lipid peroxidation or RNA damages were not the controlling mechanisms for cell death in this system. The ability of Ficoll and HEC to support cell survival during freeze-drying show that organic polymers, expected to lack the water replacing capability of e.g. disaccharides, can successfully be used as lyoprotectants. For storage under vacuum conditions we suggest that the intracellular amount of sugars (i.e. trehalose), or other protective native cell components, is sufficient for a basic protection inside the bacteria cell and that the amorphous state is the most important aspect of the formulation excipient. However, when exposed to oxygen and moisture during storage this protection is not sufficient to prevent cell degeneration.

Place, publisher, year, edition, pages
2013. Vol. 29, no 8, 1399-1408 p.
Keyword [en]
Freeze-drying, Bacteria, Organic polymers, Disaccharides, Storage stability
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-206581DOI: 10.1007/s11274-013-1303-7ISI: 000321977300007OAI: oai:DiVA.org:uu-206581DiVA: diva2:644818
Available from: 2013-09-02 Created: 2013-09-02 Last updated: 2013-09-02Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Mahlin, Denny
By organisation
Department of Pharmacy
In the same journal
World Journal of Microbiology & Biotechnology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar

Altmetric score

Total: 195 hits
ReferencesLink to record
Permanent link

Direct link