Elucidation of the BACE1 Regulating Factor GGA3 in Alzheimer's Disease
2013 (English)In: Journal of Alzheimer's Disease, ISSN 1387-2877, Vol. 37, no 1, 217-232 p.Article in journal (Refereed) Published
Golgi-localized gamma-ear-containing ADP-ribosylation factor-binding protein (GGA3) is a central regulator of trafficking and degradation of BACE1 (beta-site A beta PP-cleaving enzyme), the rate-limiting enzyme in the production of amyloid-beta (A beta) in Alzheimer's disease (AD). Here, we assessed the potential role of GGA3 in AD pathogenesis using independent neuropathological, case-control, and family-based human sample cohorts. Increased BACE1 levels coincided with decreased GGA3 levels and with elevated phosphorylation status of eIF2 alpha-Ser51 in the temporal cortex of AD patients as compared to age-matched controls. Severity of the disease did not alter mRNA or protein levels of GGA3 in the inferior temporal cortex of AD patients, while a positive correlation between GGA3 and the levels of total, but not phosphorylated, tau was observed. Genetically, we did not observe consistent evidence for association between AD risk and common GGA3 polymorphisms across a number of independent sample cohorts. However, a nominally significant association was observed with rs2242230 (p < 0.05) among the Finnish case-control cohort. Accordingly, mRNA and protein levels of GGA3 in the inferior temporal cortex of AD patients did not significantly correlate with rs2242230 genotype status. While the present study indicates that GGA3 is involved in the cellular processes relevant for AD pathogenesis, the genetic data do not support the idea that common GGA3 polymorphisms would contribute to AD risk.
Place, publisher, year, edition, pages
2013. Vol. 37, no 1, 217-232 p.
Alzheimer's disease, amyloid-beta, beta-secretase, Braak staging, eukaryotic translation initiation factor 2 alpha, frozen brain tissue samples, Golgi-localized gamma-ear-containing ADP-ribosylation factor-binding protein, polymorphism, protein stability, tau protein
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-207657DOI: 10.3233/JAD-130104ISI: 000323487300019OAI: oai:DiVA.org:uu-207657DiVA: diva2:648990