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In situ sequencing for RNA analysis in preserved tissue and cells
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Science for Life Laboratory, SciLifeLab. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Division of Visual Information and Interaction. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computerized Image Analysis and Human-Computer Interaction.
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2013 (English)In: Nature Methods, ISSN 1548-7091, E-ISSN 1548-7105, Vol. 10, no 9, 857-860 p.Article in journal (Refereed) Published
Abstract [en]

Tissue gene expression profiling is performed on homogenates or on populations of isolated single cells to resolve molecular states of different cell types. In both approaches, histological context is lost. We have developed an in situ sequencing method for parallel targeted analysis of short RNA fragments in morphologically preserved cells and tissue. We demonstrate in situ sequencing of point mutations and multiplexed gene expression profiling in human breast cancer tissue sections.

Place, publisher, year, edition, pages
2013. Vol. 10, no 9, 857-860 p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-208064DOI: 10.1038/nmeth.2563ISI: 000323760000020OAI: oai:DiVA.org:uu-208064DiVA: diva2:651080
Note

De två (2) första författarna delar förstaförfattarskapet.

Available from: 2013-09-24 Created: 2013-09-23 Last updated: 2017-12-06Bibliographically approved

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Pacureanu, AlexandraBotling, JohanWählby, CarolinaNilsson, Mats

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Department of Immunology, Genetics and PathologyScience for Life Laboratory, SciLifeLabDivision of Visual Information and InteractionComputerized Image Analysis and Human-Computer InteractionMolecular and Morphological PathologyMolecular tools
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