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Homogenization of sample absorption for the imaging of large and dense fossils with synchrotron microtomography
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Organismal Biology, Evolution and Developmental Biology.
2013 (English)In: Nature Protocols, ISSN 1754-2189, Vol. 8, no 9, 1708-1717 p.Article in journal (Refereed) Published
Abstract [en]

Propagation phase-contrast synchrotron radiation microtomography (PPC-SR mu CT) has proved to be very successful for examining fossils. Because fossils range widely in taphonomic preservation, size, shape and density, X-ray computed tomography protocols are constantly being developed and refined. Here we present a 1-h procedure that combines a filtered high-energy polychromatic beam with long-distance PPC-SR mu CT (sample to detector: 4-16 m) and an attenuation protocol normalizing the absorption profile (tested on 13-cm-thick and 5.242 g cm(-3) locally dense samples but applicable to 20-cm-thick samples). This approach provides high-quality imaging results, which show marked improvement relative to results from images obtained without the attenuation protocol in apparent transmission, contrast and signal-to-noise ratio. The attenuation protocol involves immersing samples in a tube filled with aluminum or glass balls in association with a U-shaped aluminum profiler. This technique therefore provides access to a larger dynamic range of the detector used for tomographic reconstruction. This protocol homogenizes beam-hardening artifacts, thereby rendering it effective for use with conventional mu CT scanners.

Place, publisher, year, edition, pages
2013. Vol. 8, no 9, 1708-1717 p.
National Category
Natural Sciences
URN: urn:nbn:se:uu:diva-208065DOI: 10.1038/nprot.2013.098ISI: 000323768600006OAI: oai:DiVA.org:uu-208065DiVA: diva2:651082
Available from: 2013-09-24 Created: 2013-09-23 Last updated: 2013-09-24Bibliographically approved

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