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Pathoadaptive Mutations in Salmonella enterica Isolated after Serial Passage in Mice
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology.
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 7, e70147- p.Article in journal (Refereed) Published
Abstract [en]

How pathogenic bacteria adapt and evolve in the complex and variable environment of the host remains a largely unresolved question. Here we have used whole genome sequencing of Salmonella enterica serovar Typhimurium LT2 populations serially passaged in mice to identify mutations that adapt bacteria to systemic growth in mice. We found unique pathoadaptive mutations in two global regulators, phoQ and stpA, which increase the competitive indexes of the bacteria 3- to 5-fold. Also, all mouse-adapted lineages had changed the orientation of the hin invertable element, resulting in production of a FliC type of flagellum. Competition experiments in mice with locked flagellum mutants showed that strains expressing the FliC type of flagellum had a 5-fold increase in competitive index as compared to those expressing FljB type flagellum. Combination of the flagellum cassette inversion with the stpA mutation increased competitive indexes up to 20-fold. These experiments show that Salmonella can rapidly adapt to a mouse environment by acquiring a few mutations of moderate individual effect that when combined confer substantial increases in growth.

Place, publisher, year, edition, pages
2013. Vol. 8, no 7, e70147- p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-208382DOI: 10.1371/journal.pone.0070147ISI: 000322433300090OAI: oai:DiVA.org:uu-208382DiVA: diva2:652214
Available from: 2013-09-30 Created: 2013-09-30 Last updated: 2017-12-06Bibliographically approved

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Sandegren, LinusAndersson, Dan I.

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