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Improving Precision of Proximity Ligation Assay by Amplified Single Molecule Detection
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools. Uppsala University, Science for Life Laboratory, SciLifeLab.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology, Molecular tools. Uppsala University, Science for Life Laboratory, SciLifeLab.
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2013 (English)In: PLoS ONE, ISSN 1932-6203, E-ISSN 1932-6203, Vol. 8, no 7, e69813- p.Article in journal (Refereed) Published
Abstract [en]

Proximity ligation assay (PLA) has been proven to be a robust protein detection method. The technique is characterized by high sensitivity and specificity, but the assay precision is probably limited by the PCR readout. To investigate this potential limitation and to improve precision, we developed a digital proximity ligation assay for protein measurement in fluids based on amplified single molecule detection. The assay showed significant improvements in precision, and thereby also detection sensitivity, over the conventional real-time PCR readout.

Place, publisher, year, edition, pages
2013. Vol. 8, no 7, e69813- p.
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Medical and Health Sciences
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URN: urn:nbn:se:uu:diva-208670DOI: 10.1371/journal.pone.0069813ISI: 000322064300117OAI: oai:DiVA.org:uu-208670DiVA: diva2:653955
Available from: 2013-10-07 Created: 2013-10-07 Last updated: 2017-12-06Bibliographically approved

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Nong, Rachel YuanLandegren, UlfNilsson, Mats

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