A tosyl-activated magnetic bead cellulose as solid support for sensitive protein detection
2013 (English)In: Journal of Biotechnology, ISSN 0168-1656, E-ISSN 1873-4863, Vol. 167, no 3, 235-240 p.Article in journal (Refereed) Published
Magnetic bead cellulose (MBC) was prepared using sol-gel transition of viscose in the presence of maghemite (gamma-Fe2O3) nanoparticles. The MBC particles were then activated with p-toluenesulfonyl chloride to yield tosyl-activated magnetic bead cellulose (MBC-Ts). The microspheres were characterized by light and electron microscopy, elemental analysis and atomic absorption spectroscopy to determine morphology, size, polydispersity and content of iron and tosyl groups. The functionality of the MBC-Ts microspheres was demonstrated using proximity ligation assay (PLA) to detect vascular endothelial growth factor in femtomolar concentration range. The MBC-Ts microspheres performed equally well as commercially available microparticles that are routinely used as solid support in solid phase PLA.
Place, publisher, year, edition, pages
2013. Vol. 167, no 3, 235-240 p.
Bead cellulose, Magnetic, Protein detection, Proximity ligation assay
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-208643DOI: 10.1016/j.jbiotec.2013.06.010ISI: 000324040300005OAI: oai:DiVA.org:uu-208643DiVA: diva2:654058