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The identification of the A-type RNA helices in a 55mer RNA by selective incorporation of deuterium-labelled nucleotide residues (Uppsala NMR-window concept)
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Bioorganic Chemistry.
2000 (English)In: JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, ISSN 0165-022X, Vol. 42, no 3, 153-178 p.Article in journal (Refereed) Published
Abstract [en]

The 55-nt long RNA, modelling a three-way junction, with non-uniformly incorporated deuterated nucleotides has been synthesised in a pure form. The NMR-window part in this partially deuterated 55mer RNA consists of natural non-enriched nucleotide blocks a

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2000. Vol. 42, no 3, 153-178 p.
Keyword [en]
H-2 labelling; 55mer RNA; NMR; structure; selective H-2 incorporation; NUCLEAR-MAGNETIC-RESONANCE; DEUTERATED OLIGO-DNA; HAMMERHEAD RIBOZYME; APTAMER COMPLEX; SECONDARY STRUCTURE; HETERONUCLEAR NMR; FOLDING TOPOLOGY; H-1-NMR WINDOW; RIBOSOMAL-RNA; RESOLUT
Identifiers
URN: urn:nbn:se:uu:diva-37589OAI: oai:DiVA.org:uu-37589DiVA: diva2:65488
Note
Addresses: Chattopadhyaya J, Univ Uppsala, Ctr Biomed, Dept Bioorgan Chem, Box 581, S-75123 Uppsala, Sweden. Univ Uppsala, Ctr Biomed, Dept Bioorgan Chem, S-75123 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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