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Do the 16 mer, 5 '-GUGGUCUGAUGAGGCC-3 ' and the 25 mer, 5 '-GGCCGAAACUCGUAAGAGUCACCAC-3 ', form a hammerhead ribozyme structure in physiological conditions? An NMR and UV thermodynamic study
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Bioorganic Chemistry.
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2001 (English)In: NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, ISSN 1525-7770, Vol. 20, no 4-7, 1219-1223 p.Article in journal (Refereed) Published
Abstract [en]

In a wide range of salt concentrations, 10-30 mM phosphate buffer containing up to 0.5 M Li2SO4 and 300 mM NaCl, 7.5 mM Mg2+, pH 5.5-7.5, a mixture of the 16 mer and the 25 mer RNA strands does not form a hammerhead in any amount detectable by NMR at 600

Place, publisher, year, edition, pages
MARCEL DEKKER INC , 2001. Vol. 20, no 4-7, 1219-1223 p.
Keyword [en]
H-1-NMR WINDOW; OLIGO-DNA; PROTONS; RNA
Identifiers
URN: urn:nbn:se:uu:diva-37676OAI: oai:DiVA.org:uu-37676DiVA: diva2:65575
Note
Addresses: Chatttopadhayaya J, Univ Uppsala, Ctr Biomed, Dept Bioorgan Chem, Box 581, S-75123 Uppsala, Sweden. Univ Uppsala, Ctr Biomed, Dept Bioorgan Chem, S-75123 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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