Quantification of total and visceral adipose tissue in fructose-fed rats using water-fat separated single echo MRI
2013 (English)In: Obesity, ISSN 1930-7381, E-ISSN 1930-739X, Vol. 21, no 9, E388-E395 p.Article in journal (Refereed) Published
Objective: The aim of this study was to setup a rodent model for modest weight gain and an MRI-based quantification of body composition on a clinical 1.5 T MRI system for studies of obesity and environmental factors and their possible association. Design and Methods: Twenty-four 4-week-old female Fischer rats were divided into two groups: one exposed group (n=12) and one control group (n 12). The exposed group was given drinking water containing fructose (5% for 7 weeks, then 20% for 3 weeks). The control group was given tap water. Before sacrifice, whole body MRI was performed to determine volumes of total and visceral adipose tissue and lean tissue. MRI was performed using a clinical 1.5 T system and a chemical shift based technique for separation of water and fat signal from a rapid single echo acquisition. Fat signal fraction was used to separate adipose and lean tissue. Visceral adipose tissue volume was quantified using semiautomated segmentation. After sacrifice, a perirenal fat pad and the liver were dissected and weighed. Plasma proteins were analyzed by Western blot. Results: The weight gain was 5.2% greater in rats exposed to fructose than in controls (P=0.042). Total and visceral adipose tissue volumes were 5.2 cm(3) (P=0.017) and 3.1 cm(3) (P=0.019) greater, respectively, while lean tissue volumes did not differ. The level of triglycerides and apolipoprotein A-I was higher (P=0.034, P=0.005, respectively) in fructose-exposed rats.
Place, publisher, year, edition, pages
2013. Vol. 21, no 9, E388-E395 p.
Medical Image Processing Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-209508DOI: 10.1002/oby.20229ISI: 000325426600007OAI: oai:DiVA.org:uu-209508DiVA: diva2:658149