uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
The synthetic food colouring agent Allura Red AC (E129) is not genotoxic in a flow cytometry-based micronucleus assay in vivo
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Infectious Diseases.
2013 (English)In: Food and Chemical Toxicology, ISSN 0278-6915, E-ISSN 1873-6351, Vol. 59, 86-89 p.Article in journal (Refereed) Published
Abstract [en]

The safety of several azo colouring agents, used as food additives, has during the years been questioned. Allura Red AC (E129) has in some publications been classified as genotoxic. In fact, in the European Union, Allura Red is permitted as a food additive in human food, but, surprisingly, it was not acceptable as an additive for use in animal feed. In this study we have evaluated whether Allura Red is genotoxic using a flow cytometer-based micronucleus assay in peripheral blood of mice. Male FVB mice were given a single intra-peritoneal injection of various doses of Allura Red and sacrificed at 46 h after treatment. The tested doses were 0, 100, 200, 400, 600, 800, 1000, 1500, and 2000 mg/kg body weight (b.w.). Each dose group constituted three mice, except for in the dose group of 1000 mg/kg b.w., which constituted four mice. Blood samples were collected and the frequency of micronucleated polychromatic erythrocytes (fMNPCE) and the cell proliferation (%PCE) was determined. The analyses did not show any significant difference in the %PCE or in the fMNPCE. Consequently, under the testing circumstances one can conclude that Allura Red is not genotoxic.

Place, publisher, year, edition, pages
2013. Vol. 59, 86-89 p.
Keyword [en]
Allura Red, Azo dyes, E129, Micronucleus test, Chromosome aberration
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-209486DOI: 10.1016/j.fct.2013.05.047ISI: 000324280000011OAI: oai:DiVA.org:uu-209486DiVA: diva2:658324
Available from: 2013-10-21 Created: 2013-10-21 Last updated: 2013-10-21Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Ilbäck, Nils-Gunnar
By organisation
Infectious Diseases
In the same journal
Food and Chemical Toxicology
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 229 hits
ReferencesLink to record
Permanent link

Direct link