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Chromatography on cells: analyses of solute interactions with the glucose transporter Glut1 in human red cells adsorbed on lectin-gel beads
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Biochemistry.
2000 (English)In: JOURNAL OF CHROMATOGRAPHY B, ISSN 0378-4347, Vol. 739, no 1, 55-62 p.Article in journal (Refereed) Published
Abstract [en]

The affinities of the human red cell glucose transporter Glut1 for D-glucose and cytochalasin B (CB) and the stoichiometry of CB binding vary with the Glut1 environment. In order to study the native state of Glut1 we adsorbed human red cells to wheat germ

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2000. Vol. 739, no 1, 55-62 p.
Keyword [en]
Glut1; glucose; cytochalasin B; WHEAT-GERM-AGGLUTININ; PROTEOLIPOSOME AFFINITY-CHROMATOGRAPHY; CYTOCHALASIN-B; HUMAN-ERYTHROCYTES; MEMBRANE; BINDING; RECONSTITUTION; PROTEINS
Identifiers
URN: urn:nbn:se:uu:diva-37971OAI: oai:DiVA.org:uu-37971DiVA: diva2:65870
Note
Addresses: Lundahl P, Uppsala Univ, Biomed Ctr, Dept Biochem, POB 576, SE-75123 Uppsala, Sweden. Uppsala Univ, Biomed Ctr, Dept Biochem, SE-75123 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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