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Improved heterologous expression of human glutathione transferase A4-4 by random silent mutagenesis of codons in the 5 ' region
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Chemistry, Department of Biochemistry.
2001 (English)In: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, ISSN 0304-4165, Vol. 1528, no 2-3, 101-106 p.Article in journal (Refereed) Published
Abstract [en]

Glutathione transferase A4-4 (GST A4-4) is involved in the detoxication of lipid peroxidation products such as alkenals. The human enzyme has been heterologously expressed in Escherichia coli, but for more extensive characterization of the enzyme the expr

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2001. Vol. 1528, no 2-3, 101-106 p.
Keyword [en]
glutathione transferase A4-4; heterologous expression; random silent mutagenesis; ESCHERICHIA-COLI GENES; RESPECTIVE CODONS; PROTEIN GENES; TRANSFER-RNAS; USAGE; ABUNDANCE; MUTATIONS; DESIGN; CHOICE; ENZYME
URN: urn:nbn:se:uu:diva-38029OAI: oai:DiVA.org:uu-38029DiVA: diva2:65928
Addresses: Mannervik B, Univ Uppsala, Ctr Biomed, Dept Biochem, Box 576, SE-75123 Uppsala, Sweden. Univ Uppsala, Ctr Biomed, Dept Biochem, SE-75123 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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