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Evaluating commercial MIP-columns for extraction of metoprolol with metabolites in environmental analysis
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Analytical Pharmaceutical Chemistry.
2013 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

There is of great importance to have a well suited sample preparation method when studying pharmaceutical residues in the environment, due to low concentrations and often very complex matrices. A rather newly developed solid phase for extraction is the molecularly imprinted polymers (MIP), which consists of cross linked polymers with cavities formed after shape and chemical function of a molecule. The aim of this study was to investigate a commercial available molecularly imprinted polymer cartridge (Affinilute™ MIP) for β-blockers by the use of metoprolol and two of its major metabolites, α-hydroxymetoprolol and metoprolol acid in effluent wastewater. This is, to the best of the authours knowledge, the first time the Affinilute™ MIP for β-blockers have been investigated for metabolites. The solid phase extraction (SPE) method was optimized in regard to pH, volume of sample, volume of washing solvents and type of eluent. The extraction recoveries and matrix effects of the SPE method were evaluated for the three analytes. The developed SPE method with the Affinlute™ MIP was compared to an optimized SPE method with Oasis® HLB cartridges. According to this study the molecularly imprinted polymers showed rather good results for extraction of metoprolol and the metabolite α-hydroxymetoprolol, but the Oasis® HLB cartridge was more suitable for all three of the analytes. In previous studies the use of MIP-cartridges have shown to reduce matrix effects such as ion suppression, more efficient than the HLB-cartridges, but in this study ion enhancement was observed in both cases but with less impact using HLB-cartridges.

Place, publisher, year, edition, pages
UPTEC K, ISSN 1650-8297 ; 13026
Keyword [en]
MIP, Molecularly imprinted polymers, SPE, Environmental analysis, Metoprolol
National Category
Analytical Chemistry
URN: urn:nbn:se:uu:diva-209819OAI: oai:DiVA.org:uu-209819DiVA: diva2:659667
Educational program
Master Programme in Chemical Engineering
Available from: 2013-11-19 Created: 2013-10-27 Last updated: 2013-11-19Bibliographically approved

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