In situ generation of IDA groups on nanoporous alumina for reversible immobilization of enzymes and other biomolecules.
(English)Manuscript (preprint) (Other academic)
Nanoporous alumina membranes were silanized with aminopropylsilane and iminodiacetic (IDA) groups were generated in situ by reaction with iodoacetate. The membranes were mounted in standard filter holders, connected to a HPLC system and saturated with selected metal ions. Cu(II) ions allowed the capture of chicken muscle lactate dehydrogenase with such stability, repeatability and reproducibility that Michaelis-Menten kinetics could be studied. The IDA surface was stable for months and could be depleted and regenerated with metal ions multiple times without appreciable loss of capacity. The binding of lactate dehydrogenase influenced the backpressure to the extent that could be expected for a monolayer according to Poiseuilles law.
Biochemistry and Molecular Biology
IdentifiersURN: urn:nbn:se:uu:diva-210119OAI: oai:DiVA.org:uu-210119DiVA: diva2:661110