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Real time kinetic studies of the interaction between folded antisense and target RNAs using surface plasmon resonance
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology.
2001 (English)In: JOURNAL OF MOLECULAR BIOLOGY, ISSN 0022-2836, Vol. 310, no 5, 1125-1134 p.Article in journal (Refereed) Published
Abstract [en]

Antisense RNAs interact with their complementary target RNAs as folded structures. The formation of early binding intermediates is the most important step in determining the overall rates of stable complex formation in vitro and the efficiency of control

Place, publisher, year, edition, pages
ACADEMIC PRESS LTD , 2001. Vol. 310, no 5, 1125-1134 p.
Keyword [en]
antisense RNA; surface plasmon resonance; binding kinetics; RNA-RNA interaction; binding intermediate; COLE1 ROM PROTEIN; STOICHIOMETRIC ANALYSIS; ESCHERICHIA-COLI; 4-WAY JUNCTION; STEM-LOOPS; REPLICATION; BINDING; COMPLEX; R1; COPA
Identifiers
URN: urn:nbn:se:uu:diva-38290OAI: oai:DiVA.org:uu-38290DiVA: diva2:66189
Note
Addresses: Wagner EGH, Uppsala Univ, Inst Cell & Mol Biol, Box 596, Husargatan 3, S-75124 Uppsala, Sweden. Uppsala Univ, Inst Cell & Mol Biol, S-75124 Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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