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Binding site differences revealed by crystal structures of Plasmodium falciparum and bovine acyl-CoA binding protein
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology, Structural Molecular Biology.
Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology. Uppsala University, Teknisk-naturvetenskapliga vetenskapsområdet, Faculty of Science and Technology, Biology, Department of Cell and Molecular Biology, Structural Molecular Biology.
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2001 (English)In: JOURNAL OF MOLECULAR BIOLOGY, ISSN 0022-2836, Vol. 309, no 1, 181-192 p.Article in journal (Refereed) Published
Abstract [en]

Acyl-CoA binding protein (ACBP) maintains a pool of fatty acyl-CoA molecules in the cell and plays a role in fatty acid metabolism. The biochemical properties of Plasmodium falciparum ACBP are described together with the 2.0 A resolution crystal structures of a P. falciparum ACBP-acyl-CoA complex and of bovine ACBP in two crystal forms. Overall, the bovine ACBP crystal structures are similar to the NMR structures published previously; however, the bovine and parasite ACBP structures are less similar. The parasite ACBP is shown to have a different ligand-binding pocket, leading to an acyl-CoA binding specificity different from that of bovine ACBP. Several non-conservative differences in residues that interact with the ligand were identified between the mammalian and parasite ACBPs. These, together with measured binding-specificity differences, suggest that there is a potential for the design of molecules that might selectively block the acyl-CoA binding site.

Place, publisher, year, edition, pages
2001. Vol. 309, no 1, 181-192 p.
Keyword [en]
acyl-CoA; structure; Plasmodium falciparum; drug design; X-ray crystallography; COENZYME-A; STRUCTURE REFINEMENT; 3-DIMENSIONAL STRUCTURE; LIGAND-BINDING; METABOLISM; EXPRESSION; SOFTWARE; RESIDUES; ACBP
Identifiers
URN: urn:nbn:se:uu:diva-38337OAI: oai:DiVA.org:uu-38337DiVA: diva2:66236
Available from: 2006-12-15 Created: 2006-12-15 Last updated: 2011-01-14

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Bergfors, T

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