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Paraffin section storage and immunohistochemistry - Effects of time, temperature, fixation, and retrieval protocol with emphasis on p53 protein and MIB1 antigen
Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Centre for Image Analysis. Uppsala University, Disciplinary Domain of Science and Technology, Mathematics and Computer Science, Department of Information Technology, Computerized Image Analysis.
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2000 (English)In: APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY, ISSN 1062-3345, Vol. 8, no 1, 61-70 p.Article in journal (Refereed) Published
Abstract [en]

It has been observed that immunoreactivity in paraffin sections decreased during storage. In this study, stored paraffin sections from both biopsy material and cultured cells were assessed for changes in immunoreactivity, using color-based image analysis

Place, publisher, year, edition, pages
2000. Vol. 8, no 1, 61-70 p.
Keyword [en]
paraffin section; storage; immunohistochemistry; p53 protein; MIB1 antigen; Ki-67; urinary bladder carcinoma; image analysis; CELL NUCLEAR ANTIGEN; ESTROGEN-RECEPTOR; TISSUE-SECTIONS; BREAST-CANCER; SLIDES; MICROWAVE; IMMUNOREACTIVITY; PRESERVATION; EXPRE
National Category
Computer Vision and Robotics (Autonomous Systems)
Identifiers
URN: urn:nbn:se:uu:diva-38373OAI: oai:DiVA.org:uu-38373DiVA: diva2:66272
Note
Addresses: Wester K, Univ Uppsala, Dept Genet & Pathol, Hammarskjolds 20, S-75185 Uppsala, Sweden. Univ Uppsala, Dept Genet & Pathol, S-75185 Uppsala, Sweden. Univ Uppsala, Dept Urol, S-75185 Uppsala, Sweden. Univ Uppsala, Ctr Image Anal, S-75185 Uppsala,Available from: 2005-08-25 Created: 2005-08-25 Last updated: 2015-10-21

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Ranefall, P

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