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Detection of Mycobacterium avium subsp paratuberculosis in tissue samples by single, fluorescent and nested PCR based on the IS900 gene
Uppsala University, Centre for Surface Biotechnology.
2001 (English)In: VETERINARY MICROBIOLOGY, ISSN 0378-1135, Vol. 81, no 3, 257-271 p.Article in journal (Refereed) Published
Abstract [en]

The aim of this study was to determine if fluorescent PCR could be used instead of nested PCR, for the detection of Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) in clinical specimens, to improve the sensitivity without increasing the

Place, publisher, year, edition, pages
ELSEVIER SCIENCE BV , 2001. Vol. 81, no 3, 257-271 p.
Keyword [en]
Mycobacterium avium subsp paratuberculosis; Johne's disease; diagnosis-bacteria; PCR; internal control; mimic; POLYMERASE CHAIN-REACTION; CROHNS-DISEASE TISSUE; JOHNES-DISEASE; RADIOMETRIC CULTURE; CLINICAL-SAMPLES; DNA; HYBRIDIZATION; TUBERCULOSIS; EXTRA
Identifiers
URN: urn:nbn:se:uu:diva-38386OAI: oai:DiVA.org:uu-38386DiVA: diva2:66285
Note
Addresses: Englund S, Natl Vet Inst, Dept Bacteriol, S-75189 Uppsala, Sweden. Natl Vet Inst, Dept Bacteriol, S-75189 Uppsala, Sweden. Uppsala Univ, Inst Surface Biotechnol, Uppsala, Sweden.Available from: 2008-10-17 Created: 2008-10-17 Last updated: 2011-01-14

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