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Rapid two-step purification of a recombinant mouse Fab fragment expressed in Escherichia coli
Uppsala University, Centre for Surface Biotechnology.
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2001 (English)In: PROTEIN EXPRESSION AND PURIFICATION, ISSN 1046-5928, Vol. 22, no 2, 325-329 p.Article in journal (Refereed) Published
Abstract [en]

We report a rapid, large-scale process for the purification of a recombinant Fab fragment specific for the tobacco mosaic virus coat protein (Fab57P). The fragment is expressed periplasmically in Escherichia coli. The expression level was optimized in 0.3

Place, publisher, year, edition, pages
2001. Vol. 22, no 2, 325-329 p.
Keyword [en]
MASS-TRANSPORT LIMITATION; BIOSENSOR TECHNOLOGY; BACTERIAL EXPRESSION; INTERACTION KINETICS; CHAIN; ANTIBODIES; PROTEINS
National Category
Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:uu:diva-38401OAI: oai:DiVA.org:uu-38401DiVA: diva2:66300
Note
Addresses: Janson JC, Uppsala Biomed Ctr, Ctr Surface Biotechnol, Box 577, SE-75123 Uppsala, Sweden. Uppsala Biomed Ctr, Ctr Surface Biotechnol, SE-75123 Uppsala, Sweden. Uppsala Biomed Ctr, Dept Cell & Mol Biol, SE-75124 Uppsala, Sweden. Chinese Acad SciAvailable from: 2008-06-12 Created: 2008-06-12 Last updated: 2011-01-14

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