uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
A magnetic nanobead-based bioassay provides sensitive detection of single- and biplex bacterial DNA using a portable AC susceptometer
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Nanotechnology and Functional Materials.
Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University, Solna, Sweden.
Uppsala University, Disciplinary Domain of Science and Technology, Technology, Department of Engineering Sciences, Solid State Physics.
Show others and affiliations
2014 (English)In: Biotechnology Journal, ISSN 1860-6768, E-ISSN 1860-7314, Vol. 9, no 1, 137-145 p.Article in journal (Refereed) Published
Abstract [en]

Bioassays relying on magnetic read-out using probe-tagged magnetic nanobeads are potential platforms for low-cost biodiagnostic devices for pathogen detection. For optimal assay performance it is crucial to apply an easy, efficient and robust bead-probe conjugation protocol. In this paper, sensitive (1.5 pM) singleplex detection of bacterial DNA sequences is demonstrated in a portable AC susceptometer by a magnetic nanobead-based bioassay principle; the volume-amplified magnetic nanobead detection assay (VAM-NDA). Two bead sizes, 100 and 250 nm, are investigated along with a highly efficient, rapid, robust, and stable conjugation chemistry relying on the avidin-biotin interaction for bead-probe attachment. Avidin-biotin conjugation gives easy control of the number of detection probes per bead; thus allowing for systematic investigation of the impact of varying the detection probe surface coverage upon bead immobilization in rolling circle amplified DNA-coils. The existence of an optimal surface coverage is discussed. Biplex VAM-NDA detection is for the first time demonstrated in the susceptometer: Semi-quantitative results are obtained and it is concluded that the concentration of DNA-coils in the incubation volume is of crucial importance for target quantification. The present findings bring the development of commercial biodiagnostic devices relying on the VAM-NDA further towards implementation in point-of-care and outpatient settings.

Place, publisher, year, edition, pages
2014. Vol. 9, no 1, 137-145 p.
National Category
Nano Technology
Research subject
Engineering Science with specialization in Nanotechnology and Functional Materials; Engineering Science with specialization in Solid State Physics
Identifiers
URN: urn:nbn:se:uu:diva-210682DOI: 10.1002/biot.201300348ISI: 000337540100014OAI: oai:DiVA.org:uu-210682DiVA: diva2:663992
Funder
Vinnova, 2012-00770Formas, 2011-1692 221-2012-444Carl Tryggers foundation , CTS 12:554Swedish Research Council, 2010-2580
Available from: 2013-11-13 Created: 2013-11-13 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

fulltext(968 kB)427 downloads
File information
File name FULLTEXT01.pdfFile size 968 kBChecksum SHA-512
caf639418a2d39e06d4cf972a48fc9251bbb9c53e7152039b9b2568a601ca6dce2983824d5309b7fbfadf80025625b70364f5bc1e2917a3eaf59893b09e261ca
Type fulltextMimetype application/pdf

Other links

Publisher's full text

Authority records BETA

Strömberg, MattiasZardán Gómez de la Torre, TeresaNilsson, MatsSvedlindh, PeterStrømme, Maria

Search in DiVA

By author/editor
Strömberg, MattiasZardán Gómez de la Torre, TeresaNilsson, MatsSvedlindh, PeterStrømme, Maria
By organisation
Solid State PhysicsNanotechnology and Functional Materials
In the same journal
Biotechnology Journal
Nano Technology

Search outside of DiVA

GoogleGoogle Scholar
Total: 427 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 1205 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf