Sustained release of simvastatin from premixed injectable calcium phosphate cement
2014 (English)In: Journal of Biomedical Materials Research. Part A, ISSN 1549-3296, Vol. 102, no 2, 340-347 p.Article in journal (Refereed) Published
Locally applied simvastatin is known to promote bone regeneration; however, the lack of suitable delivery systems has restricted its clinical use. In this study we demonstrate for the first time the use ofpremixed acidic calcium phosphate cement (CPC) as a delivery system for water-solubilizedsimvastatin. Freeze-dried simvastatin -hydroxy acid (SVA) was added to the premixed cement paste in four different doses (1, 0.5, 0.25, and 0 mg SVA/g cement). The addition of the drug did not alter thecement setting time (38 min), compression strength (5.54 MPa), or diametral tensile strength (2.62 MPa). In a release study conducted in phosphate buffered saline at 37 degrees C, a diffusion-controlledrelease was observed for over a week. Furthermore, the osteogenic effect of the released SVA was demonstrated in vitro. Cell proliferation, alkaline phosphatase activity, and mineralization were assayed after incubation with cement extracts. The lower doses of SVA (0.5 and 0.25 mg SVA/g cement) showed an approximately fourfold increase in mineralization as compared to the control. In conclusion, our findings suggest that premixed acidic CPC is a good option for local delivery of SVA, due to its ability of slowly releasing the drug, leading to a prolonged stimulation of osteogenesis.
Place, publisher, year, edition, pages
2014. Vol. 102, no 2, 340-347 p.
simvastatin, calcium phosphate cement, osteogenesis, drug release, Saos-2 cells
Research subject Engineering Science with specialization in Materials Science
IdentifiersURN: urn:nbn:se:uu:diva-210792DOI: 10.1002/jbm.a.34702ISI: 000328246100005OAI: oai:DiVA.org:uu-210792DiVA: diva2:664346