The cGMP-inhibitable phosphodiesterase modulates glucose transport activation by insulin
1994 (English)In: Biochimica et Biophysica Acta, ISSN 0006-3002, Vol. 1189, no 2, 163-167 p.Article in journal (Refereed) Published
To assess the role of the cGMP-inhibitable phosphodiesterase (cGI-PDE) in the action of insulin on glucose transport, adipocytes from young, lean rats were preincubated for 20 min at 37 degrees C with and without OPC 3911, a specific inhibitor of cGI-PDE, and 3-O-methylglucose uptake was measured. Insulin-stimulated glucose transport was impaired by OPC 3911 (approximately 15%) and this impairment became more pronounced in the presence of the degradable cAMP-analogue 8-bromo-cAMP (approximately 45%). This analogue alone did not significantly decrease glucose transport. Furthermore, insulin sensitivity was impaired by the combination of OPC 3911 and 8-bromo-cAMP. Maximal insulin-stimulated glucose transport in adipocytes from aging, obese rats was affected similarly by OPC 3911 and 8-bromo-cAMP, suggesting that cGI-PDE activity is not markedly altered in this insulin-resistant state. In conclusion, cGI-PDE exerts a modulating effect on the stimulatory action of insulin on glucose transport. This effect is particularly pronounced when the cellular cAMP levels are elevated.
Place, publisher, year, edition, pages
1994. Vol. 1189, no 2, 163-167 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-211335PubMedID: 8292620OAI: oai:DiVA.org:uu-211335DiVA: diva2:665999