uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Archaeological Genetics - Approaching Human History through DNA Analysis
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.
2014 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

There are a variety of archaeological questions, which are difficult to assess by traditional archaeological methods. Similarly, there are genetic and population genetic questions about human evolution and migration that are difficult to assess by studying modern day genetic variation. Archaeological genetics can directly study the archaeological remains, allowing human history to be explored by means of genetics, and genetics to be expanded into historical and pre-historical times. Examples of archaeological questions that can be resolved by genetics are determining biological sex on archaeological remains and exploring the kinship or groups buried in close proximity. Another example is one of the most important events in human prehistory – the transition from a hunter-gatherer lifestyle to farming - was driven through the diffusion of ideas or with migrating farmers. Molecular genetics has the potential to contribute in answering all these questions as well as others of similar nature. However, it is essential that the pitfalls of ancient DNA, namely fragmentation, damage and contamination are handled during data collection and data analysis.

Analyses of ancient DNA presented in this thesis are based on both mitochondrial DNA and nuclear DNA through the study of single nuclear polymorphisms (SNPs). I used pyrosequencing assays in order to identify the biological sex of archaeological remains as well as verifying if fragmented remains were human or from animal sources. I used a clonal assay approach in order to retrieve sequences for the HVRI of a small family-like burial constellation from the Viking age. By the use of low coverage shotgun sequencing I retrieved sequence data from 13 crew members from the 17th century Swedish man-of-war Kronan. This data was used to determine the ancestry of the crew, which in some cases was speculated to be of non-Scandinavian or non-European origin. However, I demonstrate that all individuals were of European ancestry. Finally, I retrieved sequence data from a Neolithic farmer from the Iberian Peninsula, which added one more facet of information in exploring the Neolithization process of Europe. The Neolithic Iberian individual was genetically similar to Scandinavian Neolithic farmers, indicating that the genetic variation of prehistoric Europe correlated with subsistence mode rather than with geography.

Place, publisher, year, edition, pages
Uppsala: Acta Universitatis Upsaliensis, 2014. , 61 p.
Series
Digital Comprehensive Summaries of Uppsala Dissertations from the Faculty of Science and Technology, ISSN 1651-6214 ; 1101
Keyword [en]
ancient DNA, pyrosequencing, molecular genetics, aDNA, neolithization, evolutionary genetics, mtDNA, viking age, archaeological genetics
Keyword [sv]
genetik, evolutionsgenetik, naturvetenskap, neolitisering, vikingatid, arkeologisk genetik
National Category
Evolutionary Biology Genetics
Research subject
Biology with specialization in Evolutionary Genetics
Identifiers
URN: urn:nbn:se:uu:diva-211156ISBN: 978-91-554-8816-1 (print)OAI: oai:DiVA.org:uu-211156DiVA: diva2:667495
Public defence
2014-03-21, Ekmansalen, EBC, Norbyvägen 14, Uppsala, 13:00 (English)
Opponent
Supervisors
Available from: 2014-03-13 Created: 2013-11-20 Last updated: 2014-04-29
List of papers
1. Further developments in molecular sex assignment: a blind test of 18th and 19th century human skeletons
Open this publication in new window or tab >>Further developments in molecular sex assignment: a blind test of 18th and 19th century human skeletons
2011 (English)In: Journal of Archaeological Science, ISSN 0305-4403, E-ISSN 1095-9238, Vol. 38, no 6, 1326-1330 p.Article in journal (Refereed) Published
Abstract [en]

The identification of sex in human remains recovered from archaeological locations is important in order to understand the social and biological structure of past societies, and to reconstruct past population demographic events. Sex determination is usually based on morphological traits of the skeletons, with the drawback that most methods do not apply to juveniles and require well preserved remains. In cases where morphological methods cannot be used, or are ambiguous, methods of molecular sexing systems are an alternative. In this methodological study we tested and validated the accuracy and usefulness of a molecular sexing method based on the amelogenin gene using pyrosequencing. We did this in a double blind study of documented 18th and 19th century human remains.

Keyword
Molecular sexing, Human, Ancient DNA, Pyrosequencing
National Category
Biological Sciences
Identifiers
urn:nbn:se:uu:diva-154599 (URN)10.1016/j.jas.2011.01.009 (DOI)000290420300016 ()
Available from: 2011-06-09 Created: 2011-06-08 Last updated: 2017-12-11Bibliographically approved
2. Mitochondrial DNA reveals a lack of kin relations in a suspected Viking age family constellation from a Swedish proto-Christian burial site.
Open this publication in new window or tab >>Mitochondrial DNA reveals a lack of kin relations in a suspected Viking age family constellation from a Swedish proto-Christian burial site.
(English)Manuscript (preprint) (Other academic)
Keyword
ancient DNA, genetics, mtDNA, kinskip, Sweden, Viking, Estrid, aDNA
National Category
Archaeology Genetics
Identifiers
urn:nbn:se:uu:diva-211151 (URN)
Available from: 2013-11-20 Created: 2013-11-20 Last updated: 2014-04-29
3. Genomic analysis of biogeographic ancestry of 15 crewmembers from the 300 year-old Swedish man-of-war Kronan
Open this publication in new window or tab >>Genomic analysis of biogeographic ancestry of 15 crewmembers from the 300 year-old Swedish man-of-war Kronan
Show others...
(English)Manuscript (preprint) (Other academic)
Keyword
Marine archaeology, Kronan, ancient DNA, genetics
National Category
Archaeology Genetics Evolutionary Biology
Identifiers
urn:nbn:se:uu:diva-211152 (URN)
Available from: 2013-11-20 Created: 2013-11-20 Last updated: 2014-04-29
4. A late Neolithic Iberian farmer exhibits genetic affinity to Neolithic Scandinavian farmers and a Bronze Age central European  farmer
Open this publication in new window or tab >>A late Neolithic Iberian farmer exhibits genetic affinity to Neolithic Scandinavian farmers and a Bronze Age central European  farmer
Show others...
(English)Manuscript (preprint) (Other academic)
Keyword
ancient DNA, neolithization, Iberia, Portalon, aDNA, SNP
National Category
Archaeology Evolutionary Biology Genetics
Identifiers
urn:nbn:se:uu:diva-211155 (URN)
Available from: 2013-11-20 Created: 2013-11-20 Last updated: 2014-04-29

Open Access in DiVA

fulltext(53351 kB)2444 downloads
File information
File name FULLTEXT01.pdfFile size 53351 kBChecksum SHA-512
39b81dc6a6d0b70187c4d763f2b64e0646ba2d8f4537516b86017ec04a1a1402f5899bab1858b33f3f659ba1a8ef04a284296fddad6a491f8f87c60039b615af
Type fulltextMimetype application/pdf
Buy this publication >>

Authority records BETA

Daskalaki, Evangelia

Search in DiVA

By author/editor
Daskalaki, Evangelia
By organisation
Evolutionary Biology
Evolutionary BiologyGenetics

Search outside of DiVA

GoogleGoogle Scholar
Total: 2444 downloads
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

isbn
urn-nbn

Altmetric score

isbn
urn-nbn
Total: 977 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf