Evaluation of α-tocopherols effect on catechol and o-phenylenediamine induced DNA damage: An in vitro study using two different staining techniques in the comet assay
(English)Article in journal (Refereed) Submitted
Using the alkaline comet assay, the effect of a physiologically relevant concentration of a-tocopherol (20 mM) on the DNA damaging effects of catechol and o-phenylenedi- amine (OPD) was evaluated in mouse lymphoma L5178Y TK+/- cells. For compara- tive purposes, a third mutagenic agent, 4-nitro-o-phenylenediamine (4-NOPD), was also included in the study. a-Tocopherol was found to be without DNA damaging effects of its own after three hours exposure when tested in concentrations up to 500 mM, and this vitamin was also found to significantly reduce the DNA damage induced by 1 mM catechol. However, a-tocopherol did not reduce the level of DNA damage induced by OPD, indicating that catechol and OPD induce DNA damage by different mechanisms of action. As in a previous study, 4-NOPD did not increase the level of DNA damage, and a-tocopherol did not affect the level of damage when the cells were concomitantly exposed to both agents. The results from a separate ex- periment using different staining techniques for cells that had been exposed to dif- ferent concentrations of catechol, clearly indicated that the more hazardous chemical ethidium bromide could be substituted with the less hazardous chemical GelRedTM in the comet assay.
α-tocopherol, catechol, OPD, DNA damage, comet assay, mouse lymphoma cells, GelRed
Pharmacology and Toxicology
Research subject Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-211514OAI: oai:DiVA.org:uu-211514DiVA: diva2:674271