Cellular and functional specificity among ferritin-like proteins in the multicellular cyanobacterium Nostoc punctiforme.
2014 (English)In: Environmental Microbiology, ISSN 1462-2912, E-ISSN 1462-2920, Vol. 16, no 3, 829-844 p.Article in journal (Refereed) Published
Ferritin-like proteins constitute a remarkably heterogeneous protein family, including ferritins, bacterioferritins and Dps proteins. The genome of the filamentous heterocyst-forming cyanobacterium Nostoc punctiforme encodes five ferritin-like proteins. In the present paper, we report a multidimensional characterization of these proteins. Our phylogenetic and bioinformatics analyses suggest both structural and physiological differences among the ferritin-like proteins. The expression of these five genes responded differently to hydrogen peroxide treatment, with a significantly higher rise in transcript level for Npun_F3730 as compared with the other four genes. A specific role for Npun_F3730 in the cells tolerance against hydrogen peroxide was also supported by the inactivation of Npun_F3730, Npun_R5701 and Npun_R6212; among these, only the ΔNpun_F3730 strain showed an increased sensitivity to hydrogen peroxide compared with wild type. Analysis of promoter-GFP reporter fusions of the ferritin-like genes indicated that Npun_F3730 and Npun_R5701 were expressed in all cell types of a diazotrophic culture, while Npun_F6212 was expressed specifically in heterocysts. Our study provides the first comprehensive analysis combining functional differentiation and cellular specificity within this important group of proteins in a multicellular cyanobacterium.
Place, publisher, year, edition, pages
Oxford: Wiley-Blackwell, 2014. Vol. 16, no 3, 829-844 p.
Natural Sciences Microbiology
IdentifiersURN: urn:nbn:se:uu:diva-212373DOI: 10.1111/1462-2920.12233ISI: 000332085400017PubMedID: 23992552OAI: oai:DiVA.org:uu-212373DiVA: diva2:677391