Analysis of neuropeptides by MALDI imaging mass spectrometry
2013 (English)In: Methods in Molecular Biology, ISSN 1064-3745, Vol. 1023, 121-136 p.Article in journal (Refereed) Published
Matrix-assisted laser desorption ionization (MALDI) imaging mass spectrometry (IMS) is one of the most effective tools for localizing small molecules and compounds directly in thin tissue sections. MALDI IMS should be used when the distribution of molecular species is not known and to localize changes due to a disease process or a treatment. In recent years it has become increasingly clear that many pathological processes are not readily correlated to dramatic changes in protein levels. MALDI IMS can aid the localization of areas where the cellular concentration of proteins may be high enough to play an important biological role, but when the precise location is unknown. Here, we present a MALDI IMS protocol and data analysis of molecular imaging of multiple rat brain sections.
Place, publisher, year, edition, pages
2013. Vol. 1023, 121-136 p.
Research subject Neuroscience
IdentifiersURN: urn:nbn:se:uu:diva-212977DOI: 10.1007/978-1-4614-7209-4_7PubMedID: 23765622OAI: oai:DiVA.org:uu-212977DiVA: diva2:680033
FunderSwedish Research Council