uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
No in vitro effects of fatty acids on glucose uptake, lipolysis or insulin signaling in rat adipocytes.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Sciences, Endocrinology, Diabetes and Metabolism.
2004 (English)In: Hormone and Metabolic Research, ISSN 0018-5043, E-ISSN 1439-4286, Vol. 36, no 4, 203-9 p.Article in journal (Refereed) Published
Abstract [en]

Elevated plasma levels of free fatty acids (FFA) can produce insulin resistance in skeletal muscle tissue and liver and, together with alterations in beta-cell function, this has been referred to as lipotoxicity. This study explores the effects of FFAs on insulin action in rat adipocytes. Cells were incubated 4 or 24 h with or without an unsaturated FFA, oleate or a saturated FFA, palmitate (0.6 and 1.5 mM, respectively). After the culture period, cells were washed and insulin effects on glucose uptake and lipolysis as well as cellular content of insulin signaling proteins (IRS-1, PI3-kinase, PKB and phosphorylated PKB) and the insulin regulated glucose transporter GLUT4 were measured. No significant differences were found in basal or insulin-stimulated glucose uptake in FFA-treated cells compared to control cells, regardless of fatty acid concentration or incubation period. Moreover, there were no significant alterations in the expression of IRS-1, PI3-kinase, PKB and GLUT4 following FFA exposure. Insulin's ability to stimulate PKB phosphorylation was also left intact. Nor did we find any alterations following FFA exposure in basal or cAMP-stimulated lipolysis or in the ability of insulin to inhibit lipolysis. The results indicate that oleate or palmitate does not directly influence insulin action to stimulate glucose uptake and inhibit lipolysis in rat fat cells. Thus, lipotoxicity does not seem to occur in the fat tissue itself.

Place, publisher, year, edition, pages
2004. Vol. 36, no 4, 203-9 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-211291DOI: 10.1055/s-2004-814446PubMedID: 15114517OAI: oai:DiVA.org:uu-211291DiVA: diva2:681588
Available from: 2013-12-20 Created: 2013-11-21 Last updated: 2013-12-20

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed
By organisation
Endocrinology, Diabetes and Metabolism
In the same journal
Hormone and Metabolic Research
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 127 hits
ReferencesLink to record
Permanent link

Direct link