Hydroxyapatite coating affects the Wnt signaling pathway during peri-implant healing in vivo
2014 (English)In: Acta Biomaterialia, ISSN 1742-7061, Vol. 10, no 3, 1451-1462 p.Article in journal (Refereed) Published
Owing to its bio- and osteoconductivity, hydroxyapatite (HA) is a widely used implant material, but its osteogenic properties are only partly evaluated in vitro and in vivo. The present study focused on bone healing adjacent to HA-coated titanium (Ti) implants, with or without incorporated lithium ions (Li+). Special attention was given to the Wnt signaling pathway. The implants were inserted into rat tibia for 7 or 28 days and analyzed ex vivo, mainly by histomorphometry and quantitative real-time polymerase chain reaction (qPCR). HA-coated implants showed, irrespective of Li+ content, bone-implant contact (BIC) and removal torque values significantly higher than those of reference Ti. Further, the expression of OCN, CTSK, COL1A1, LRP5/6 and WISP1 was significantly higher in implant-adherent cells of HA-coated implants, with or without Li+. Significantly higher beta-catenin expression and significantly lower COL2A1 expression were observed in pen-implant bone cells from HA with 14 ng cm(-2) released Li+. Interestingly, Ti implants showed a significantly larger bone area (BA) in the threads than HA with 39 ng cm(-2) released Li+, but had a lower BIC than any HA-coated implant. This study shows that HA, with or without Li+, is a strong activator of the Wnt signaling pathway, and may to some degree explain its high bone induction capacity.
Place, publisher, year, edition, pages
2014. Vol. 10, no 3, 1451-1462 p.
Biomaterials Science Engineering and Technology
Research subject Engineering Science with specialization in Materials Science
IdentifiersURN: urn:nbn:se:uu:diva-213615DOI: 10.1016/j.actbio.2013.12.012ISI: 000331663900039OAI: oai:DiVA.org:uu-213615DiVA: diva2:682919
FunderSwedish Research Council, K20125-2X-094952-53 6212-0116-037