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Establishment and characterization of a primary and a metastatic melanoma cell line from Grey horses
Dept Forens Sci, A-1090 Vienna, Austria;Med Univ Vienna, Ctr Physiol & Pharmacol, Vascobiolab, A-1090 Vienna, Austria; Univ Vienna, Dept Pharmacol & Toxicol, A-1090 Vienna, Austria.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Medical Biochemistry and Microbiology. Uppsala University, Science for Life Laboratory, SciLifeLab.
Med Univ Vienna, Dept Cell Biol & Ultrastruct Res, Ctr Anat & Cell Biol, A-1090 Vienna, Austria.
Med Univ Vienna, Inst Immunol, A-1090 Vienna, Austria.
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2014 (English)In: In vitro Cellular & Developmental Biology-Animal, ISSN 1071-2690, E-ISSN 1543-706X, Vol. 50, no 1, 56-65 p.Article in journal (Refereed) Published
Abstract [en]

The Grey horse phenotype, caused by a 4.6 kb duplication in Syntaxin 17, is strongly associated with high incidence of melanoma. In contrast to most human melanomas with an early onset of metastasis, the Grey horse melanomas have an extended period of benign growth, after which 50% or more eventually undergo progression and may metastasize. In efforts to define changes occurring during Grey horse melanoma progression, we established an in vitro model comprised of two cell lines, HoMel-L1 and HoMel-A1, representing a primary and a metastatic stage of the melanoma, respectively. The cell lines were examined for their growth and morphological characteristics, in vitro and in vivo oncogenic potential, chromosome numbers, and expression of melanocytic antigens and tumor suppressors. Both cell lines exhibited malignant characteristics; however, the metastatic HoMel-A1 showed a more aggressive phenotype characterized by higher proliferation rates, invasiveness, and a stronger tumorigenic potential both in vitro and in vivo. HoMel-A1 displayed a near-haploid karyotype, whereas HoMel-L1 was near-diploid. The cell lines expressed melanocytic lineage markers such as TYR, TRP1, MITF, PMEL, ASIP, MC1R, POMC, and KIT. The tumor suppressor p53 was strongly expressed in both cell lines, while the tumor suppressors p16 and PTEN were absent in HoMel-A1, potentially implicating significance of these pathways in the melanoma progression. This in vitro model system will not only aid in understanding of the Grey horse melanoma pathogenesis, but also in unraveling the steps during melanoma progression in general as well as being an invaluable tool for development of new therapeutic strategies.

Place, publisher, year, edition, pages
2014. Vol. 50, no 1, 56-65 p.
Keyword [en]
Grey horse, Primary and metastatic melanoma, Cell lines, In vitro model, Tumor suppressor
National Category
Cell Biology Cell Biology
Identifiers
URN: urn:nbn:se:uu:diva-215428DOI: 10.1007/s11626-013-9678-1ISI: 000330806300007PubMedID: 23982913OAI: oai:DiVA.org:uu-215428DiVA: diva2:687189
Funder
Swedish Cancer Society, CAN 2011/703
Available from: 2014-01-13 Created: 2014-01-13 Last updated: 2017-12-06Bibliographically approved

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Sundström, ElisabethAndersson, LeifGolovko, Anna

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