Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels
2005 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 244, 385-390 p.Article in journal (Refereed) Published
DNA from environmental PCR products separated by denaturing gradient gel electrophoresis (DGGE) was isolated from the background smear rather than from discrete bands of the DGGE gel. The ‘‘interband’’ region was considered as a potential source of less dominant members of natural microbial communities. Surprisingly, instead of detecting new bands from the re-amplified PCR products, patterns very similar to the original ones were obtained regardless of the position of the ‘‘interband’’ region. The results suggest that the separation of amplicons by DGGE may not be perfect and band re-amplification based sequence analyses need careful interpretation.
Place, publisher, year, edition, pages
2005. Vol. 244, 385-390 p.
IdentifiersURN: urn:nbn:se:uu:diva-215581DOI: 10.1016/j.femsle.2005.02.013ISI: 000227860400023OAI: oai:DiVA.org:uu-215581DiVA: diva2:687656