uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Observation of bias associated with re-amplification of DNA isolated from denaturing gradient gels
Department of Microbiology, Eötvös Loránd University of Science, Budapest, Hungary.
Show others and affiliations
2005 (English)In: FEMS Microbiology Letters, ISSN 0378-1097, E-ISSN 1574-6968, Vol. 244, 385-390 p.Article in journal (Refereed) Published
Abstract [en]

DNA from environmental PCR products separated by denaturing gradient gel electrophoresis (DGGE) was isolated from the background smear rather than from discrete bands of the DGGE gel. The ‘‘interband’’ region was considered as a potential source of less dominant members of natural microbial communities. Surprisingly, instead of detecting new bands from the re-amplified PCR products, patterns very similar to the original ones were obtained regardless of the position of the ‘‘interband’’ region. The results suggest that the separation of amplicons by DGGE may not be perfect and band re-amplification based sequence analyses need careful interpretation.

Place, publisher, year, edition, pages
2005. Vol. 244, 385-390 p.
National Category
Microbiology
Identifiers
URN: urn:nbn:se:uu:diva-215581DOI: 10.1016/j.femsle.2005.02.013ISI: 000227860400023OAI: oai:DiVA.org:uu-215581DiVA: diva2:687656
Available from: 2014-01-14 Created: 2014-01-14 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Szekely, Anna

Search in DiVA

By author/editor
Szekely, Anna
In the same journal
FEMS Microbiology Letters
Microbiology

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 367 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf