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Subtyping of gliomas of various WHO grades by the application of immunohistochemistry
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Radiology, Oncology and Radiation Science.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Immunology, Genetics and Pathology.
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2014 (English)In: Histopathology, ISSN 0309-0167, E-ISSN 1365-2559, Vol. 64, no 3, 365-379 p.Article in journal (Refereed) Published
Abstract [en]

Aims

In 2010, four subtypes (classical, proneural, mesenchymal, and neural) of glioblastoma multiforme (GBM) were defined by molecular genetic analyses. The objective of this study was to assess whether gliomas, independently of the type and grade, could be subdivided into protein-based subtypes.

Methods and results

A tissue microarray (TMA) approach was applied to incorporate tissue samples of low-grade and high-grade gliomas into five TMAs. High expression levels of epidermal growth factor receptor (EGFR), CD44, c-MER proto-oncogene tyrosine kinase (MERTK), platelet-derived growth factor receptor α, p53, oligodendrocyte transcription factor 2 (OLIG2) and isocitrate dehydrogenase 1 with the R132H mutation were assessed using immunohistochemistry (IHC). Glioma could be subdivided into four subtypes by IHC. The majority of the low-grade gliomas were of the proneural subtype, i.e. high p53 expression (63% of grade II). The classical subtype, with high EGFR and low p53 expression, was most common in GBMs (39%), followed by the proneural (29%) and mesenchymal (with high CD44 and MERTK expression) (29%) subtypes, a frequency that is in line with previously published data based on molecular genetics.

Conclusions

Assessment of the expression of the five proteins EGFR, CD44, MERTK, p53 and OLIG2 is sufficient for subtyping gliomas, and can be recommended for implementation in clinical practice for both low-grade and high-grade gliomas.

Place, publisher, year, edition, pages
2014. Vol. 64, no 3, 365-379 p.
National Category
Medical and Health Sciences
Identifiers
URN: urn:nbn:se:uu:diva-215836DOI: 10.1111/his.12252ISI: 000330638700005OAI: oai:DiVA.org:uu-215836DiVA: diva2:688620
Available from: 2014-01-17 Created: 2014-01-17 Last updated: 2017-12-06Bibliographically approved

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Popova, Svetlana NBergqvist, MichaelDimberg, AnnaEdqvist, Per-HenrikEkman, SimonHesselager, GöranPonten, FredrikSmits, AnjaSooman, LindaAlafuzoff, Irina

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Popova, Svetlana NBergqvist, MichaelDimberg, AnnaEdqvist, Per-HenrikEkman, SimonHesselager, GöranPonten, FredrikSmits, AnjaSooman, LindaAlafuzoff, Irina
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Department of Immunology, Genetics and PathologyDepartment of Radiology, Oncology and Radiation ScienceOncologyNeurosurgeryMolecular and Morphological PathologyNeurology
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Histopathology
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