uu.seUppsala University Publications
Change search
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Accurate sex identification of ancient human remains using DNA shotgun sequencing
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology.
Uppsala University, Disciplinary Domain of Science and Technology, Biology, Department of Ecology and Genetics, Evolutionary Biology. Uppsala University, Science for Life Laboratory, SciLifeLab.
2013 (English)In: Journal of Archaeological Science, ISSN 0305-4403, E-ISSN 1095-9238, Vol. 40, no 12, 4477-4482 p.Article in journal (Refereed) Published
Abstract [en]

Accurate identification of the biological sex of ancient remains is vital for critically testing hypotheses about social structure in prehistoric societies. However, morphological methods are imprecise for juvenile individuals and fragmentary remains, and molecular methods that rely on particular sex-specific marker loci such as the amelogenin gene suffer from allelic dropout and sensitivity to modern contamination. Analyzing shotgun sequencing data from 14 present-day humans of known biological sex and 16 ancient individuals from a time span of 100 to similar to 70,000 years ago, we show that even relatively sparse shotgun sequencing (about 100,000 human sequences) can be used to reliably identify chromosomal sex simply by considering the ratio of sequences aligning to the X and Y chromosomes, and highlight two examples where the genetic assignments indicate morphological misassignment Furthermore, we show that accurate sex identification of highly degraded remains can be performed in the presence of substantial amounts of present-day contamination by utilizing the signature of cytosine deamination, a characteristic feature of ancient DNA.

Place, publisher, year, edition, pages
2013. Vol. 40, no 12, 4477-4482 p.
Keyword [en]
Ancient DNA, Sex identification, DNA analysis, Genomics, Shotgun sequencing
National Category
Natural Sciences
Identifiers
URN: urn:nbn:se:uu:diva-215929DOI: 10.1016/j.jas.2013.07.004ISI: 000328015000032OAI: oai:DiVA.org:uu-215929DiVA: diva2:689087
Available from: 2014-01-20 Created: 2014-01-17 Last updated: 2017-12-06Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Authority records BETA

Skoglund, PontusJakobsson, Mattias

Search in DiVA

By author/editor
Skoglund, PontusJakobsson, Mattias
By organisation
Evolutionary BiologyScience for Life Laboratory, SciLifeLab
In the same journal
Journal of Archaeological Science
Natural Sciences

Search outside of DiVA

GoogleGoogle Scholar

doi
urn-nbn

Altmetric score

doi
urn-nbn
Total: 450 hits
CiteExportLink to record
Permanent link

Direct link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf