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Immunohistochemical analysis of dynorphin B levels in the mouse substantia nigra after single doses of cocaine and morphine.
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Pharmaceutical Biosciences.
2014 (English)Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

Abstract The basal ganglia plays an essential part in the generation of reward, motor control, development of habits, learning and drug addiction. The main neurotransmitter related to natural and drug induced reward and the development of drug addiction is dopamine. Opioid neuropeptides such as dynorphins and enkephalins are important components in drug abuse. They regulate dopamine and affect the activity of the reward system. Therefore the aim of this study was to analyze the levels of dynorphins and enkephalins with MALDI IMS in the substantia nigra and ventral tegmental area of mice treated with a single dose of cocaine or morphine. Thin coronal sections of mouse brains were made using a cryostat-microtome. The sections were then analyzed using MALDI IMS and double fluorescent immunohistochemistry.

No significant changes in dynorphin B levels in mice receiveing a single dose of morphine or cocaine 15 and 240 minutes before sacrifice could be shown in this study. Data indicated a release of dynorphin B in the substantia nigra, 15 minutes after a cocaine or morphine injection; also a normalization of dynorphin levels was seen after 240 minutes. Morphine and cocaine are two very common drugs of abuse; morphine is also commonly used in medicine. This makes it essential to understand the initial effects of these drugs on the brain, in order to make medical treatment and treatment against drug abuse safer and more effective.

Place, publisher, year, edition, pages
2014. , 27 p.
National Category
Pharmacology and Toxicology
URN: urn:nbn:se:uu:diva-216463OAI: oai:DiVA.org:uu-216463DiVA: diva2:689917
Subject / course
Educational program
Master of Science Programme in Pharmacy
Available from: 2014-05-28 Created: 2014-01-22 Last updated: 2014-05-28Bibliographically approved

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