uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Affinity analysis of lectin interaction with immobilized C- and O- gylcosides studied by surface plasmon resonance assay
Institute of Chemistry, Slovak Academy of Sciences, Dúbravská cesta 9, SK-84238, Bratislava, Slovak Republic.
Show others and affiliations
2002 (English)In: Journal of Biochemical and Biophysical Methods, ISSN 0165-022X, E-ISSN 1872-857X, Vol. 52, no 1, 11-18 p.Article in journal (Refereed) Published
Abstract [en]

A biosensor based on the surface plasmon resonance (SPR) principle was used for kinetic analysis of lectin interactions with different immobilized saccharide structures. A novel affinity ligands beta-D-glycopyranosylmethylamines derived from common D-aldohexoses linked to the carboxymethyl dextran layer of the SPR sensor surface served for interactions with a wide range of lectins. The method of preparation and use of the beta-D-mannopyranosyl glycosylated sensor surface was described. The results of affinity analysis of lectin-ligand interactions were evaluated and compared with data obtained from measurements using commercially available p-aminophenyl alpha-D-glycopyranosides. Possible applications and advantages of C- and O-glycosylated SPR biosensors are discussed.

Place, publisher, year, edition, pages
2002. Vol. 52, no 1, 11-18 p.
National Category
Biological Sciences
URN: urn:nbn:se:uu:diva-216575DOI: 10.1016/S0165-022X(02)00016-7PubMedID: 12121750OAI: oai:DiVA.org:uu-216575DiVA: diva2:690365
Available from: 2014-01-23 Created: 2014-01-23 Last updated: 2014-01-23Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full textPubMed

Search in DiVA

By author/editor
Nahálková, Jarmila
In the same journal
Journal of Biochemical and Biophysical Methods
Biological Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 166 hits
ReferencesLink to record
Permanent link

Direct link