Protein bodies in the pine megagametophyte in vitro culture: ultrastructural, histochemical and electrophoretic observations
2000 (English)In: Biologia plantarum, ISSN 0006-3134, E-ISSN 1573-8264, Vol. 43, no 3, 329-336 p.Article in journal (Refereed) Published
The megagametophytes of the European black pine (Pinus nigra Am.) were cultured on modified MS medium. After 10 d, protein bodies showed well-marked degradation on freeze-etched replicas and in preparations observed by scanning electron microscopy. After 20 d of cultivation, the megagametophyte cells were completely empty. Proteins secreted into the agar medium were determined by electrophoresis and 15 different proteins, in the range of 6.5 to 71 kDa, were identified.
Place, publisher, year, edition, pages
2000. Vol. 43, no 3, 329-336 p.
IdentifiersURN: urn:nbn:se:uu:diva-216582DOI: 10.1023/A:1026795506674ISI: 000089279700002OAI: oai:DiVA.org:uu-216582DiVA: diva2:690370