Defense reaction of pine seeds: distribution of protein bodies extract from Pinus nigra seeds in agar medium plates
1999 (English)In: Biologia (Bratislava), ISSN 0006-3088, E-ISSN 1336-9563, Vol. 54, no 1, 107-111 p.Article in journal (Refereed) Published
Diffusion and distribution of protein bodies (PB) extract from Pinus nigra seeds in agar medium plates was studied. After application of PB extract on filter paper discs it was placed on agar plates for 20 days. Proteins diffused from the discs were distributed in the plates. Identical protein pattern (8-54 kDa molecules) of PB extract was defined by SDS-PAGE analysis in agar strips at different distances from the discs. The predominant peptides and proteins were 8, 9, 13 and 18 kDa. The 32-36 kDa proteins were absent. These results suggest that the proteins during the maintenance on agar medium plates were separated. Relative quantity of individual proteins was different in certain distances from the discs. It is probable that these proteins can be involved in defense of pine megagametophyte. We assume that proportion of individual proteins is also of importance in their defense function.
Place, publisher, year, edition, pages
1999. Vol. 54, no 1, 107-111 p.
IdentifiersURN: urn:nbn:se:uu:diva-216586ISI: 000079863800015OAI: oai:DiVA.org:uu-216586DiVA: diva2:690376