Development of an in vitro model for evaluating the pharmacological effects of new formulations of insulin and GLP-1
Independent thesis Advanced level (professional degree), 20 credits / 30 HE creditsStudent thesis
BACKGROUND: Metabolic disorders, such as obesity and diabetes, are an increasing problem in the world nowadays. The treatment of diabetes needs improvements in order to make more efficient pharmaceutical formulations for oral intake and sustained drug release. Micro- and nano-technology is a growing field and have potential for different applications, also in diabetes treatment. Porous silicon (PSi) is a material used with great potential in the treatment of several diseases, and the advantages of this material are its biodegradability and biocompatibility in the body. AIM: The aim of this study was to develop an in vitro model for evaluating the pharmacological effects of new formulations of insulin and Glucagon-like peptide 1 (GLP-1). METHOD: Three different cell lines where used and different settings tested for the in vitro model. Glucose- and insulin- concentrations were the parameters used to evaluate the pharmacological effects. RESULTS: The results show that the glucose concentrations seem to increase in the cells when stimulating with insulin. Also the insulin secretion increased when stimulating a pancreatic cell line with GLP-1. It also seems that there is no difference in response between different cell lines. The variations between replicates make it rather difficult to draw any conclusions. CONCLUSIONS: The results show that the in vitro model needs more development in order to get the right settings for further application with micro or nanomaterials. Improvements of the cell culture methods and comparison of the glucose assay with other methods for intracellular glucose uptake are also needed to be considered in the future.
Place, publisher, year, edition, pages
2014. , 40 p.
Pharmacology and Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-216631OAI: oai:DiVA.org:uu-216631DiVA: diva2:690554
Master of Science Programme in Pharmacy
Raasmaja, AtsoSantos, Hélder A.