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Transient OGG1, APE1, PARP1 and Pol beta expression in an Alzheimer's disease mouse model
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2013 (English)In: Mechanisms of Ageing and Development, ISSN 0047-6374, E-ISSN 1872-6216, Vol. 134, no 10, 467-477 p.Article in journal (Refereed) Published
Abstract [en]

Alzheimer's disease (AD) is a disease of major public health significance, whose pathogenesis is strongly linked to the presence of fibrillar aggregates of amyloid-beta (A beta) in the aging human brain. We exploited the transgenic (Tg)-ArcSwe mouse model for human AD to explore whether oxidative stress and the capacity to repair oxidative DNA damage via base excision repair (BER) are related to A beta pathology in AD. Tg-ArcSwe mice express variants of A beta, accumulating senile plaques at 4-6 months of age, and develop AD-like neuropathology as adult animals. The relative mRNA levels of genes encoding BER enzymes, including 8-oxoguanine glycosylase (OGG1), AP endonuclease 1 (APE1), polymerase 13 (Pol beta) and poly(ADP-ribose) polymerase 1 (PARP1), were quantified in various brain regions of 6 weeks, 4 months and 12 months old mice. The results show that OGG1 transcriptional expression was higher, and APE1 expression lower, in 4 months old Tg-ArcSwe than in wildtype (wt) mice. Furthermore, Pol beta transcriptional expression was significantly lower in transgenic 12 months old mice than in wt. Transcriptional profiling also showed that BER repair capacity vary during the lifespan in Tg-ArcSwe and wt mice. The BER expression pattern in Tg-ArcSwe mice thus reflects responses to oxidative stress in vulnerable brain structures. 

Place, publisher, year, edition, pages
2013. Vol. 134, no 10, 467-477 p.
Keyword [en]
DNA repair, Base excision repair (BER), Alzheimer's disease, A beta PP mutation, Transgenic (Tg-ArcSwe) mice, OGG1, APE1, PARP1, Pol beta
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-216889DOI: 10.1016/j.mad.2013.09.002ISI: 000329148400006OAI: oai:DiVA.org:uu-216889DiVA: diva2:691279
Available from: 2014-01-27 Created: 2014-01-27 Last updated: 2014-01-27Bibliographically approved

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Nilsson, Lars N. G.
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