Effekter av frivilligt alkoholintag på my-opioida receptordensiteten och förekomst av lateralisering i råtthjärna
Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Background: The biological mechanism for alcohol use disorders (AUD) remains unclear but genetic predisposition, in combination with environmental factors are involved in the development of AUD. The role of the endogenous opioid system in alcohol consumption has diligently been studied for decades and it is known that the mu opioid receptor (MOPR) is central for reward and addiction. The reinforcing effects of alcohol seem to increase if it simultaneously interacts with the MOPR and inhibition of the MOPR has resulted in decreased alcohol intake. Furthermore, the right hemisphere has shown to be more sensitive to alcohol compared to the left, indicating that anatomical and functional asymmetries appear between the two hemispheres. It is therefore of interest to investigate how voluntary alcohol intake affects the MOPR and also study if there are asymmetries in receptor density between the left and right hemispheres. Methods: Wistar male rats were introduced to ethanol by a two-bottle free- choice paradigm. The rats had intermittent 24-hour access to 20 % ethanol for three consecutive days a week for seven weeks. The rats were sacrificed 10-11 days after the final ethanol intake in order to investigate long-term effects in the brain. Later, the brains were sectioned into 12 μm slices at six different bregma levels. An autoradiographic method was used with [3H]DAMGO and the MOPR density was measured with densiometric methods. Results: Ethanol-drinking rats showed different MOPR density in the nucleus accumbens core and shell, hippocampus and substantia nigra relative to water-drinking rats. A cortical area showed altered MOPR density on the left hemisphere relative to the right regardless of intake. Finally, in a limbic area a significant interaction in MOPR density between intake groups and left and right hemisphere was found. Conclusion: Voluntary intake of 20 % ethanol can cause long-term changes in brain structures of importance for reward and addiction and affect the two hemispheres differently.
Place, publisher, year, edition, pages
2014. , 39 p.
farmakologi, neurofarmakologi, beroende, alkohol
Pharmacology and Toxicology
IdentifiersURN: urn:nbn:se:uu:diva-216965OAI: oai:DiVA.org:uu-216965DiVA: diva2:691469
Subject / course
Master of Science Programme in Pharmacy