Serial Femtosecond Crystallography of G Protein-Coupled Receptors
2013 (English)In: Science, ISSN 0036-8075, E-ISSN 1095-9203, Vol. 342, no 6165, 1521-1524 p.Article in journal (Refereed) Published
X-ray crystallography of G protein-coupled receptors and other membrane proteins is hampered by difficulties associated with growing sufficiently large crystals that withstand radiation damage and yield high-resolution data at synchrotron sources. We used an x-ray free-electron laser (XFEL) with individual 50-femtosecond-duration x-ray pulses to minimize radiation damage and obtained a high-resolution room-temperature structure of a human serotonin receptor using sub-10-micrometer microcrystals grown in a membrane mimetic matrix known as lipidic cubic phase. Compared with the structure solved by using traditional microcrystallography from cryo-cooled crystals of about two orders of magnitude larger volume, the room-temperature XFEL structure displays a distinct distribution of thermal motions and conformations of residues that likely more accurately represent the receptor structure and dynamics in a cellular environment.
Place, publisher, year, edition, pages
2013. Vol. 342, no 6165, 1521-1524 p.
Medical and Health Sciences
IdentifiersURN: urn:nbn:se:uu:diva-217630DOI: 10.1126/science.1244142ISI: 000328644300062OAI: oai:DiVA.org:uu-217630DiVA: diva2:694294