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Methods for radiolabelling of monoclonal antibodies
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Radiology, Oncology and Radiation Science, Biomedical Radiation Sciences. (Tolmachev)
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Pharmacy, Department of Medicinal Chemistry, Preclinical PET Platform.ORCID iD: 0000-0001-6120-2683
Uppsala University, Disciplinary Domain of Medicine and Pharmacy, Faculty of Medicine, Department of Radiology, Oncology and Radiation Science, Biomedical Radiation Sciences.
2014 (English)In: Human Monoclonal Antibodies: Methods and Protocols / [ed] Michael Steinitz, Humana Press, 2014, 309-330 p.Chapter in book (Refereed)
Abstract [en]

The use of radionuclide labels allows to study the pharmacokinetics of monoclonal antibodies, to control the specificity of their targeting and to monitor the response to an antibody treatment with high accuracy. Selection of label depends on the processing of an antibody after binding to an antigen, and on the character of information to be derived from the study (distribution of antibody in the extracellular space, target occupancy or determination of sites of metabolism). This chapter provides protocols for labelling of antibodies with iodine-125 (suitable also for other radioisotopes of iodine) and with indium-111. Since radiolabelling might damage and reduce the immunoreactive fraction and/or affinity of an antibody, the methods for assessment of these characteristics of an antibody are provided for control.

Place, publisher, year, edition, pages
Humana Press, 2014. 309-330 p.
Series
Methods in Molecular Biology, ISSN 1064-3745 ; 1060
National Category
Radiology, Nuclear Medicine and Medical Imaging
Identifiers
URN: urn:nbn:se:uu:diva-218570DOI: 10.1007/978-1-62703-586-6_16PubMedID: 24037848ISBN: 978-1-62703-585-9 (print)ISBN: 978-1-62703-586-6 (print)OAI: oai:DiVA.org:uu-218570DiVA: diva2:695997
Available from: 2014-02-12 Created: 2014-02-12 Last updated: 2015-03-24Bibliographically approved

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Tolmachev, VladimirOrlova, AnnaAndersson, Karl

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