uu.seUppsala University Publications
Change search
ReferencesLink to record
Permanent link

Direct link
Pseudomonas aeruginosa Disrupts Caenorhabditis elegans Iron Homeostasis, Causing a Hypoxic Response and Death
Uppsala University, Science for Life Laboratory, SciLifeLab.
Show others and affiliations
2013 (English)In: Cell Host and Microbe, ISSN 1931-3128, Vol. 13, no 4, 406-416 p.Article in journal (Refereed) Published
Abstract [en]

The opportunistic pathogen Pseudomonas aeruginosa causes serious human infections, but effective treatments and the mechanisms mediating pathogenesis remain elusive. Caenorhabditis elegans shares innate immune pathways with humans, making it invaluable to investigate infection. To determine how P. aeruginosa disrupts host biology, we studied how P. aeruginosa kills C. elegans in a liquid-based pathogenesis model. We found that P. aeruginosa-mediated killing does not require quorum-sensing pathways or host colonization. A chemical genetic screen revealed that iron chelators alleviate P. aeruginosa-mediated killing. Consistent with a role for iron in P. aeruginosa pathogenesis, the bacterial siderophore pyoverdin was required for virulence and was sufficient to induce a hypoxic response and death in the absence of bacteria. Loss of the C. elegans hypoxia-inducing factor HIF-1, which regulates iron homeostasis, exacerbated P. aeruginosa pathogenesis, further linking hypoxia and killing. As pyoverdin is indispensable for virulence in mice, pyoverdin-mediated hypoxia is likely to be relevant in human pathogenesis.

Place, publisher, year, edition, pages
2013. Vol. 13, no 4, 406-416 p.
National Category
Medical and Health Sciences
URN: urn:nbn:se:uu:diva-219887DOI: 10.1016/j.chom.2013.03.003ISI: 000330850500006OAI: oai:DiVA.org:uu-219887DiVA: diva2:703491
Available from: 2014-03-07 Created: 2014-03-06 Last updated: 2014-03-07Bibliographically approved

Open Access in DiVA

No full text

Other links

Publisher's full text

Search in DiVA

By author/editor
Wählby, Carolina
By organisation
Science for Life Laboratory, SciLifeLab
In the same journal
Cell Host and Microbe
Medical and Health Sciences

Search outside of DiVA

GoogleGoogle Scholar
The number of downloads is the sum of all downloads of full texts. It may include eg previous versions that are now no longer available

Altmetric score

Total: 134 hits
ReferencesLink to record
Permanent link

Direct link